just to clarify:
Alicia+Beth are dead due to Priscilla killing them in the west.
Alicia+Beth were the first successful pair of Awakening Twins
(Rafaela+Luciela were the prototype Awakening Twins, but they weren't successful at actually becoming Awakening Twins).
these are NEW TRAINEE (and pretty young too) Awakening Twins.
-----------------------------------------------------------------------------------
to monad, small correction:
the Org sent another "batch" of AFs/AEs along with Alicia+Beth to kill Riful, as the other AFs/AEs were still gobbling up what few scraps of meat of Isley was left, hehe.
we never learn what happens to those AFs/AEs whom gobbled up Isley.
this is because, the AFs/AEs have to be trained to hunt a specific scent. the AFs/AEs whom were gobbling up Isley, were only trained to hunt down Isley.
they needed new/other AFs/AEs trained to hunt down Riful's scent, to hunt down Riful for Alicia+Beth to finish her-Riful off themselves. the AFs/AEs were Alicia+Beth 's "bloodhounds".
-----------------------------------------------------------------
However, those AFs/AEs were doing quite well against Riful+Dauf on their own, lol, before Alicia+Beth arrived shortly behind them.
Though, the AFs/AEs mainly were doing well, because Riful had to actually use her eyes to physically see them, as they didn't give off yoki for her to sense. This is how they managed to get sneak-attack-munches out off of her body, lol.
(this is also how the 4 HA Ghosts survived their encounter with Riful as they rescued Audrey+Rachel, since their yokis couldn't be sensed by Riful due to their NYS*)
(this is also how the 7 Ghosts decimated Agatha so easily as well, due to their NYS*)
*NYS = Natural Yoki Supression = this occurs from not using yoki for years = the 7 Ghosts (Miria, Cynthia, Deneve, Helen, Tabatha, Yuma, and Clare) in hiding, Irene in hiding, and Rafaela in exile/banishiment.